eng-GB is thought to be the default language - all the text pieces used in the templates are in eng-GB and then translated into the target language using the i18n operator. That's why there is no eng-GB translation file by default. And if I read the code in i18n.php correctly the text translation is disabled if there is no file for the language or the fallback languages (if any).
I am not sure why you think that the eng-US file should be checked - none of the settings in your site.ini suggests so. The untranslated file is, as far as I know. only there to make creating new translations easier.
Greetings from Luxembourg
Claudia
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